PrimePCR™ Probe Assay: CREB3L4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CREB3L4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CREB3L4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052408
Assay Design:   exonic
Chromosome Location:   1:153941823-153941912question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000427349 ENST00000460112 ENST00000376020 ENST00000377942 ENST00000338862 ENST00000262418 ENST00000294304 ENST00000449724 ENST00000368607 ENST00000271889 ENST00000368601 ENST00000368603 ENST00000368600 ENST00000431292 ENST00000405694 ENST00000366697 ENST00000366698 ENST00000295033 ENST00000600171 ENST00000596464

Gene Information

This gene encodes a CREB (cAMP responsive element binding) protein with a transmembrane domain which localizes it to the ER membrane. The encoded protein is a transcriptional activator which contains a dimerization domain and this protein may function in a number of processing pathways including protein processing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Dec 2011]

Gene Symbol:   CREB3L4
Gene Name:   cAMP responsive element binding protein 3-like 4
Aliases:   AIBZIP, ATCE1, CREB3, CREB4, JAL, hJAL
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000143578
Entrez:   148327
Chromosome Mapping:   1q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.830000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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