PrimePCR™ Probe Assay: CRNN, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052402
Assay Design:   exonic
Chromosome Location:   1:152382161-152382272question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000271835

Gene Information

This gene encodes a member of the "fused gene" family of proteins which contain N-terminus EF-hand domains and multiple tandem peptide repeats. The encoded protein contains two EF-hand Ca2+ binding domains in its N-terminus and two glutamine- and threonine-rich 60 amino acid repeats in its C-terminus. This gene also known as squamous epithelial heat shock protein 53 may play a role in the mucosal/epithelial immune response and epidermal differentiation. [provided by RefSeq Jan 2009]

Gene Symbol:   CRNN
Gene Name:   cornulin
Aliases:   C1orf10, DRC1, PDRC1, SEP53
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000143536
Entrez:   49860
UniGene:   Hs.242057
Chromosome Mapping:   1q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.790000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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