PrimePCR™ Probe Assay: TAF1A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TAF1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TAF1A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052398
Assay Design:   exonic
Chromosome Location:   1:222750816-222750961question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000366890 ENST00000352967 ENST00000391883 ENST00000350027 ENST00000391882 ENST00000543857

Gene Information

This gene encodes a subunit of the RNA polymerase I complex Selectivity Factor I (SLI). The encoded protein is a TATA box-binding protein-associated factor that plays a role in the assembly of the RNA polymerase I preinitiation complex. Alternate splicing results in multiple transcript variants encoding multiple isoforms.[provided by RefSeq Jan 2011]

Gene Symbol:   TAF1A
Gene Name:   TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kDa
Aliases:   MGC:17061, RAFI48, SL1, TAFI48
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000143498
Entrez:   9015
Chromosome Mapping:   1q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.590000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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