PrimePCR™ Probe Assay: TMCO1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TMCO1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TMCO1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052359
Assay Design:   exonic
Chromosome Location:   1:165697348-165712518question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000392129 ENST00000367881 ENST00000580248 ENST00000464650 ENST00000481278

Gene Information

This locus encodes a transmembrane protein. Mutations at this locus have been associated with craniofacial dysmorphism skeletal anomalies and mental retardation. Mutations at this locus have also been associated with open angle glaucoma blindness. Alternatively spliced transcript variants have been described. [provided by RefSeq Jan 2012]

Gene Symbol:   TMCO1
Gene Name:   transmembrane and coiled-coil domains 1
Aliases:   HP10122, PCIA3, PNAS-136, RP11-466F5.7, TMCC4
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000143183
Entrez:   54499
Chromosome Mapping:   1q22-q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 36.320000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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