PrimePCR™ Probe Assay: SERBP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052340
Assay Design:   exonic
Chromosome Location:   1:67878687-67878827question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000370994 ENST00000370995 ENST00000361219 ENST00000370990

Gene Information

Description Not Available

Gene Symbol:   SERBP1
Gene Name:   SERPINE1 mRNA binding protein 1
Aliases:   CHD3IP, DKFZp564M2423, FLJ90489, HABP4L, PAI-RBP1, PAIRBP1
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000142864
Entrez:   26135
UniGene:   Hs.530412
Chromosome Mapping:   1p31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.450000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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