PrimePCR™ Probe Assay: ZNF787, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ZNF787, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF787, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052318
Assay Design:   exonic
Chromosome Location:   19:56600440-56632545question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000371884 ENST00000294339 ENST00000371883 ENST00000448023 ENST00000359128 ENST00000301749 ENST00000419350 ENST00000270459 ENST00000586787 ENST00000587279 ENST00000529064 ENST00000425753 ENST00000529048 ENST00000584601 ENST00000578199 ENST00000584450 ENST00000269571 ENST00000578709 ENST00000584099 ENST00000541774 ENST00000406381 ENST00000540147 ENST00000540042 ENST00000445067 ENST00000369744 ENST00000466741 ENST00000524472 ENST00000497220 ENST00000369748 ENST00000343257 ENST00000428304 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF787
Gene Name:   zinc finger protein 787
Aliases:   TIP20
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000142409
Entrez:   126208
Chromosome Mapping:   19q13.43

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.440000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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