PrimePCR™ Probe Assay: CBX4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052293
Assay Design:   exonic
Chromosome Location:   17:77809117-77809458question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000446488 ENST00000524794 ENST00000524746 ENST00000304521 ENST00000396912 ENST00000495122 ENST00000448310 ENST00000269397

Gene Information

Description Not Available

Gene Symbol:   CBX4
Gene Name:   chromobox homolog 4
Aliases:   NBP16, PC2
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000141582
Entrez:   8535
UniGene:   Hs.730763
Chromosome Mapping:   17q25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.110000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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