PrimePCR™ Probe Assay: DCAF5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052205
Assay Design:   exonic
Chromosome Location:   14:69558518-69583087question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000341516 ENST00000554215 ENST00000556847 ENST00000557386 ENST00000389997 ENST00000554681

Gene Information

Description Not Available

Gene Symbol:   DCAF5
Gene Name:   DDB1 and CUL4 associated factor 5
Aliases:   BCRG2, BCRP2, D14S1461E, DKFZp434A035, KIAA1824, WDR22
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000139990
Entrez:   8816
UniGene:   Hs.509780
Chromosome Mapping:   14q23-q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 35.300000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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