PrimePCR™ Probe Assay: SLC7A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052177
Assay Design:   exonic
Chromosome Location:   13:30109990-30110139question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000380752 ENST00000450494

Gene Information

Description Not Available

Gene Symbol:   SLC7A1
Gene Name:   solute carrier family 7 (cationic amino acid transporter, y+ system), member 1
Aliases:   ATRC1, CAT-1, ERR, HCAT1, REC1L
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000139514
Entrez:   6541
UniGene:   Hs.14846
Chromosome Mapping:   13q12-q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.440000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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