PrimePCR™ Probe Assay: GCOM1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GCOM1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GCOM1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052057
Assay Design:   exonic
Chromosome Location:   15:57921905-57922023question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000334815 ENST00000261609 ENST00000395123 ENST00000395124 ENST00000352187 ENST00000529554 ENST00000362021 ENST00000354884 ENST00000524928 ENST00000518533 ENST00000359441 ENST00000379936 ENST00000238788 ENST00000404032 ENST00000296424 ENST00000504285 ENST00000509245 ENST00000268489 ENST00000397992 ENST00000436639 ENST00000402813 ENST00000514170 ENST00000420489 ENST00000544810 ENST00000358519 ENST00000371666 ENST00000345254 ENST00000339365 ENST00000316637 ENST00000380569 ENST00000587652 ENST

Gene Information

This locus represents naturally occurring readthrough transcription between the neighboring MYZAP (myocardial zonula adherens protein) and POLR2M (polymerase (RNA) II (DNA directed) polypeptide M) genes on chromosome 15. Alternate splicing results in two readthrough transcript variants that encode different isoforms. One of the readthrough variants encodes a fusion protein that shares sequence identity with each individual gene product. The other variant encodes a protein that shares sequence identity with the upstream gene product but its C-terminal region is distinct due to frameshifts relative to the downstream gene. [provided by RefSeq Nov 2011]

Gene Symbol:   GCOM1
Gene Name:   GRINL1A complex locus
Aliases:   FLJ30973, GRINL1A, Gcom2, Gup, Gup1, Gup2, MGC126694, MGC138353, MYOZAP
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000137878
Entrez:   145781
Chromosome Mapping:   15q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.880000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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