PrimePCR™ Probe Assay: TTPA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052031
Assay Design:   exonic
Chromosome Location:   8:63973725-63973854question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000260116

Gene Information

This gene encodes a soluble protein that binds alpha-trocopherol a form of vitamin E with high selectivity and affinity. This protein plays an important role in regulating vitamin E levels in the body by transporting vitamin E between membrane vesicles and facilitating the secretion of vitamin E from hepatocytes to circulating lipoproteins. Mutations in this gene cause hereditary vitamin E deficiency (ataxia with vitamin E deficiency AVED) and retinitis pigmentosa. [provided by RefSeq Nov 2009]

Gene Symbol:   TTPA
Gene Name:   tocopherol (alpha) transfer protein
Aliases:   ATTP, AVED, TTP1, alphaTTP
RefSeq:   NC_000008.10 NG_016123.1 NT_008183.19
Ensembl:   ENSG00000137561
Entrez:   7274
UniGene:   Hs.69049
Chromosome Mapping:   8q12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.630000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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