PrimePCR™ Probe Assay: CPEB2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052026
Assay Design:   exonic
Chromosome Location:   4:15055757-15060054question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000507071 ENST00000345451 ENST00000259997 ENST00000382395 ENST00000382401 ENST00000509684 ENST00000538197 ENST00000541112 ENST00000442003

Gene Information

The protein encoded by this gene is highly similar to cytoplasmic polyadenylation element binding protein (CPEB) an mRNA-binding protein that regulates cytoplasmic polyadenylation of mRNA as a trans factor in oogenesis and spermatogenesis. Studies of the similar gene in mice suggested a possible role of this protein in transcriptionally inactive haploid spermatids. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   CPEB2
Gene Name:   cytoplasmic polyadenylation element binding protein 2
Aliases:   CPE-BP2, CPEB-2, MGC119575, MGC119576, MGC119577, hCPEB-2
RefSeq:   NC_000004.11 NT_006316.16
Ensembl:   ENSG00000137449
Entrez:   132864
UniGene:   Hs.656937
Chromosome Mapping:   4p15.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.440000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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