PrimePCR™ Probe Assay: CNPY3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052012
Assay Design:   exonic
Chromosome Location:   6:42905568-42905914question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000372836 ENST00000394142

Gene Information

PRAT4A is associated with the immature form of TLR4 (MIM 603030) and regulates its cell surface expression (Wakabayashi et al. 2006 [PubMed 16849487]).[supplied by OMIM Mar 2008]

Gene Symbol:   CNPY3
Gene Name:   canopy 3 homolog (zebrafish)
Aliases:   CAG4A, ERDA5, PRAT4A, TNRC5
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000137161
Entrez:   10695
UniGene:   Hs.414099
Chromosome Mapping:   6pter-p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.620000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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