PrimePCR™ Probe Assay: NCBP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051996
Assay Design:   exonic
Chromosome Location:   9:100431138-100431250question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000375147 ENST00000375130

Gene Information

The product of this gene is a component of the nuclear cap-binding protein complex (CBC) which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing 3'-end processing RNA nuclear export and nonsense-mediated mRNA decay. [provided by RefSeq Jul 2008]

Gene Symbol:   NCBP1
Gene Name:   nuclear cap binding protein subunit 1, 80kDa
Aliases:   CBP80, MGC2087, NCBP, Sto1
RefSeq:   NC_000009.11 NG_011642.1 NT_008470.19
Ensembl:   ENSG00000136937
Entrez:   4686
UniGene:   Hs.595669
Chromosome Mapping:   9q34.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.910000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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