PrimePCR™ Probe Assay: SLC31A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051987
Assay Design:   exonic
Chromosome Location:   9:115983864-116018396question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000374212 ENST00000374210

Gene Information

The protein encoded by this gene is a high-affinity copper transporter found in the cell membrane. The encoded protein functions as a homotrimer to effect the uptake of dietary copper. [provided by RefSeq Aug 2011]

Gene Symbol:   SLC31A1
Gene Name:   solute carrier family 31 (copper transporters), member 1
Aliases:   COPT1, CTR1, MGC75487, hCTR1
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000136868
Entrez:   1317
UniGene:   Hs.532315
Chromosome Mapping:   9q31-q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.610000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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