PrimePCR™ Probe Assay: SMPD4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SMPD4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SMPD4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051970
Assay Design:   exonic
Chromosome Location:   2:130911345-130911488question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000403733 ENST00000323319 ENST00000361669 ENST00000439827 ENST00000394720 ENST00000444444 ENST00000294868 ENST00000287713 ENST00000374232 ENST00000317968 ENST00000503974 ENST00000514743 ENST00000437932 ENST00000542407 ENST00000358024 ENST00000330675 ENST00000329800 ENST00000397335 ENST00000542125 ENST00000574561 ENST00000320345 ENST00000406424 ENST00000421807 ENST00000351288 ENST00000439886 ENST00000409031 ENST00000457039 ENST00000449159 ENST00000431183 ENST00000453750 ENST00000339679 ENST

Gene Information

Sphingomyelinases (EC 3.1.4.12) such as SMPD4 catalyze the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide (Krut et al. 2006 [PubMed 16517606]).[supplied by OMIM Mar 2008]

Gene Symbol:   SMPD4
Gene Name:   sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3)
Aliases:   FLJ20297, FLJ20756, KIAA1418, NET13, NSMASE-3, NSMASE3
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000136699
Entrez:   55627
Chromosome Mapping:   2q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.830000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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