PrimePCR™ Probe Assay: ERMN, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051963
Assay Design:   exonic
Chromosome Location:   2:158181951-158182074question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000410096 ENST00000397283 ENST00000420719 ENST00000420317 ENST00000411762 ENST00000409216 ENST00000409925 ENST00000419116

Gene Information

Description Not Available

Gene Symbol:   ERMN
Gene Name:   ermin, ERM-like protein
Aliases:   JN, KIAA1189, ermin
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000136541
Entrez:   57471
UniGene:   Hs.443894
Chromosome Mapping:   2q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.850000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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