PrimePCR™ Probe Assay: BZW2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051941
Assay Design:   exonic
Chromosome Location:   7:16714081-16714198question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000415365 ENST00000258761 ENST00000405202 ENST00000446596 ENST00000438834 ENST00000430000 ENST00000433922 ENST00000452975

Gene Information

Description Not Available

Gene Symbol:   BZW2
Gene Name:   basic leucine zipper and W2 domains 2
Aliases:   MST017, MSTP017
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000136261
Entrez:   28969
UniGene:   Hs.487635
Chromosome Mapping:   7p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 37.130000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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