PrimePCR™ Probe Assay: CHST5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CHST5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CHST5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051888
Assay Design:   exonic
Chromosome Location:   16:75564035-75564183question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000398249 ENST00000222330 ENST00000279034 ENST00000300591 ENST00000579038 ENST00000441551 ENST00000398722 ENST00000375442 ENST00000252137 ENST00000237654 ENST00000567020 ENST00000293970 ENST00000564879 ENST00000434757 ENST00000254977 ENST00000399006 ENST00000405977 ENST00000378226 ENST00000432825 ENST00000159111 ENST00000536461 ENST00000588361 ENST00000344113 ENST00000554584 ENST00000358025 ENST00000357395 ENST00000297338 ENST00000523986 ENST00000518055 ENST00000336257 ENST00000565039 ENST

Gene Information

The protein encoded by this gene belongs to the Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST) family members of which catalyze the transfer of sulfate to position 6 of galactose (Gal) N-acetylgalactosamine (GalNAc) or N-acetylglucosamine (GlcNAc) residues within proteoglycans and sulfation of O-linked sugars of mucin-type acceptors. Carbohydrate sulfation plays a critical role in many biologic processes. This gene is predominantly expressed in colon and small intestine. [provided by RefSeq Aug 2011]

Gene Symbol:   CHST5
Gene Name:   carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5
Aliases:   I-GlcNAc-6-ST, I-GlcNAc6ST, MGC74625, glcNAc6ST-3, gn6st-3, hIGn6ST
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000135702
Entrez:   23563
Chromosome Mapping:   16q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.960000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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