PrimePCR™ Probe Assay: TFCP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051866
Assay Design:   exonic
Chromosome Location:   12:51511503-51512475question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000257915 ENST00000549867 ENST00000548115 ENST00000548108 ENST00000307660

Gene Information

This gene encodes a transcription factor that binds the alpha-globin promoter and activates transcription of the alpha-globin gene. The encoded protein regulates erythroid gene expression plays a role in the transcriptional switch of globin gene promoters and it activates many other cellular and viral gene promoters. The gene product interacts with certain inflammatory response factors and polymorphisms of this gene may be involved in the pathogenesis of Alzheimer's disease. [provided by RefSeq Mar 2010]

Gene Symbol:   TFCP2
Gene Name:   transcription factor CP2
Aliases:   LBP1C, LSF, LSF1D, SEF, TFCP2C
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000135457
Entrez:   7024
UniGene:   Hs.48849
Chromosome Mapping:   12q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.720000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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