PrimePCR™ Probe Assay: DNAJC14, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051863
Assay Design:   exonic
Chromosome Location:   12:56222256-56222372question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000357606 ENST00000317287 ENST00000547445 ENST00000546957 ENST00000317269

Gene Information

Description Not Available

Gene Symbol:   DNAJC14
Gene Name:   DnaJ (Hsp40) homolog, subfamily C, member 14
Aliases:   DNAJ, DRIP78, HDJ3, LIP6
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000135392
Entrez:   85406
UniGene:   Hs.709320
Chromosome Mapping:   12q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.440000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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