PrimePCR™ Probe Assay: ISCA1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051850
Assay Design:   exonic
Chromosome Location:   9:88880609-88880706question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000375991 ENST00000311534 ENST00000452279

Gene Information

ISCA1 is a mitochondrial protein involved in the biogenesis and assembly of iron-sulfur clusters which play a role in electron-transfer reactions (Cozar-Castellano et al. 2004 [PubMed 15262227]).[supplied by OMIM Mar 2008]

Gene Symbol:   ISCA1
Gene Name:   iron-sulfur cluster assembly 1 homolog (S. cerevisiae)
Aliases:   HBLD2, ISA1, MGC4276, RP11-507D14.2, hIscA
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000135070
Entrez:   81689
UniGene:   Hs.449291
Chromosome Mapping:   9q21.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.200000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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