PrimePCR™ Probe Assay: FST, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051810
Assay Design:   exonic
Chromosome Location:   5:52780866-52780961question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000256759 ENST00000396947 ENST00000504226 ENST00000497789

Gene Information

Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms FST317 and FST344 containing 317 and 344 amino acids respectively resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families evidence was found for linkage between PCOS and follistatin. [provided by RefSeq Jul 2008]

Gene Symbol:   FST
Gene Name:   follistatin
Aliases:   FS
RefSeq:   NC_000005.9 NT_006713.15
Ensembl:   ENSG00000134363
Entrez:   10468
UniGene:   Hs.9914
Chromosome Mapping:   5q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.310000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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