PrimePCR™ Probe Assay: SPIRE1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SPIRE1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SPIRE1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051808
Assay Design:   exonic
Chromosome Location:   18:12535505-12546674question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000409402 ENST00000410092 ENST00000309836 ENST00000453447 ENST00000449797 ENST00000497844 ENST00000462226 ENST00000383356

Gene Information

Spire proteins such as SPIRE1 are highly conserved between species. They belong to the family of Wiskott-Aldrich homology region-2 (WH2) proteins which are involved in actin organization (Kerkhoff et al. 2001 [PubMed 11747823]).[supplied by OMIM Mar 2008]

Gene Symbol:   SPIRE1
Gene Name:   spire homolog 1 (Drosophila)
Aliases:   MGC150621, MGC150622, Spir-1
RefSeq:   NC_000018.9 NT_010859.14
Ensembl:   ENSG00000134278
Entrez:   56907
Chromosome Mapping:   18p11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.350000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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