PrimePCR™ Probe Assay: IER3IP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051789
Assay Design:   exonic
Chromosome Location:   18:44681632-44681738question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000256433

Gene Information

This gene encodes a small protein that is localized to the endoplasmic reticulum (ER) and may play a role in the ER stress response by mediating cell differentiation and apoptosis. Transcription of this gene is regulated by tumor necrosis factor alpha and specificity protein 1 (Sp1). Mutations in this gene may play a role in microcephaly epilepsy and diabetes syndrome (MEDS) and a pseudogene of this gene is located on the long arm of chromosome 12. [provided by RefSeq Dec 2011]

Gene Symbol:   IER3IP1
Gene Name:   immediate early response 3 interacting protein 1
Aliases:   PRO2309
RefSeq:   NC_000018.9 NT_010966.14
Ensembl:   ENSG00000134049
Entrez:   51124
UniGene:   Hs.130917
Chromosome Mapping:   18q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.880000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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