PrimePCR™ Probe Assay: HSD17B4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051774
Assay Design:   exonic
Chromosome Location:   5:118814615-118824902question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000256216 ENST00000515320 ENST00000510025 ENST00000504811 ENST00000414835 ENST00000513628 ENST00000509514

Gene Information

The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. [provided by RefSeq Jul 2008]

Gene Symbol:   HSD17B4
Gene Name:   hydroxysteroid (17-beta) dehydrogenase 4
Aliases:   DBP, MFE-2, MPF-2, SDR8C1
RefSeq:   NC_000005.9 NG_008182.1 NT_034772.6
Ensembl:   ENSG00000133835
Entrez:   3295
UniGene:   Hs.406861
Chromosome Mapping:   5q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.720000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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