PrimePCR™ Probe Assay: BEX2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051753
Assay Design:   exonic
Chromosome Location:   X:102564455-102564604question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000321702 ENST00000472042 ENST00000257951 ENST00000372677 ENST00000372674 ENST00000536889 ENST00000342219 ENST00000412904 ENST00000393684

Gene Information

This gene belongs to the brain expressed X-linked gene family. The encoded protein interacts with the transcription factor LIM domain only 2 in a DNA-binding complex that recognizes the E-box element and promotes transcription. This gene has been found to be a tumor suppressor that is silenced in human glioma. In breast cancer cells this gene product modulates apoptosis in response to estrogen and tamoxifen and enhances the anti-proliferative effect of tamoxifen. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Dec 2009]

Gene Symbol:   BEX2
Gene Name:   brain expressed X-linked 2
Aliases:   BEX1, DJ79P11.1
RefSeq:   NC_000023.10 NT_011651.17 NG_016254.1
Ensembl:   ENSG00000133134
Entrez:   84707
UniGene:   Hs.398989
Chromosome Mapping:   Xq22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.880000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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