PrimePCR™ Probe Assay: CRP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051721
Assay Design:   exonic
Chromosome Location:   1:159683831-159684217question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000255030 ENST00000368112 ENST00000368111 ENST00000368110 ENST00000343919

Gene Information

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently the level of this protein in plasma increases greatly during acute phase response to tissue injury infection or other inflammatory stimuli. [provided by RefSeq Sep 2009]

Gene Symbol:   CRP
Gene Name:   C-reactive protein, pentraxin-related
Aliases:   MGC149895, MGC88244, PTX1
RefSeq:   NC_000001.10 NG_013007.1 NT_004487.19
Ensembl:   ENSG00000132693
Entrez:   1401
UniGene:   Hs.76452
Chromosome Mapping:   1q21-q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.160000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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