PrimePCR™ Probe Assay: VPS25, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051650
Assay Design:   exonic
Chromosome Location:   17:40931022-40931136question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000253794 ENST00000589520

Gene Information

VPS25 VPS36 (MIM 610903) and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II) a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25 VPS36 and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al. 2005 [PubMed 15755741]; Kamura et al. 2001 [PubMed 11278625]).[supplied by OMIM Mar 2008]

Gene Symbol:   VPS25
Gene Name:   vacuolar protein sorting 25 homolog (S. cerevisiae)
Aliases:   DERP9, EAP20, FAP20, MGC10540
RefSeq:   NC_000017.10 NG_016227.1 NT_010783.15
Ensembl:   ENSG00000131475
Entrez:   84313
UniGene:   Hs.500165
Chromosome Mapping:   17q21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.570000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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