PrimePCR™ Probe Assay: DUSP9, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051607
Assay Design:   exonic
Chromosome Location:   X:152915502-152915592question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000547291 ENST00000308385 ENST00000279488 ENST00000357814 ENST00000580301 ENST00000429709 ENST00000450518 ENST00000392662 ENST00000495880 ENST00000296483 ENST00000225916 ENST00000370167 ENST00000433144 ENST00000342782 ENST00000371897 ENST00000371899 ENST00000356575 ENST00000440172 ENST00000422774 ENST00000242365 ENST00000290722 ENST00000375371 ENST00000567240 ENST00000563527 ENST00000564173 ENST00000267064 ENST00000347471 ENST00000550164 ENST00000394023 ENST00000595095 ENST00000601432 ENST

Gene Information

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK SAPK/JNK p38) which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases different tissue distribution and subcellular localization and different modes of inducibility of their expression by extracellular stimuli. This gene product shows selectivity for members of the ERK family of MAP kinases is expressed only in placenta kidney and fetal liver and is localized to the cytoplasm and nucleus. [provided by RefSeq Jul 2008]

Gene Symbol:   DUSP9
Gene Name:   dual specificity phosphatase 9
Aliases:   MKP-4, MKP4
RefSeq:   NC_000023.10 NT_167198.1
Ensembl:   ENSG00000130829
Entrez:   1852
UniGene:   Hs.144879
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.010000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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