PrimePCR™ Probe Assay: APOC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: APOC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: APOC1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051561
Assay Design:   exonic
Chromosome Location:   19:45418199-45419529question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000298466 ENST00000425225 ENST00000588750 ENST00000588802 ENST00000592535 ENST00000592885 ENST00000589078 ENST00000586638 ENST00000252491 ENST00000373468 ENST00000055682

Gene Information

The protein encoded by this gene is a member of the apolipoprotein C1 family. This gene is expressed primarily in the liver and it is activated when monocytes differentiate into macrophages. A pseudogene of this gene is located 4 kb downstream in the same orientation on the same chromosome. This gene is mapped to chromosome 19 where it resides within a apolipoprotein gene cluster. Alternatively spliced transcript variants have been found for this gene but the biological validity of some variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   APOC1
Gene Name:   apolipoprotein C-I
Aliases:   Not Available
RefSeq:   NC_000019.9 NG_012859.1 NT_011109.16
Ensembl:   ENSG00000130208
Entrez:   341
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.920000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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