PrimePCR™ Probe Assay: GDPD2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051554
Assay Design:   exonic
Chromosome Location:   X:69649396-69649489question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000374382 ENST00000453994 ENST00000536730 ENST00000538649

Gene Information

This gene encodes a member of the glycerophosphodiester phosphodiesterase enzyme family. The encoded protein hydrolyzes glycerophosphoinositol to produce inositol 1-phosphate and glycerol. This protein may have a role in osteoblast differentiation and growth. Alternate splicing results in multiple transcript variants. [provided by RefSeq Jan 2010]

Gene Symbol:   GDPD2
Gene Name:   glycerophosphodiester phosphodiesterase domain containing 2
Aliases:   FLJ20207, GDE3, OBDPF
RefSeq:   NC_000023.10 NT_011669.17 NG_016413.1
Ensembl:   ENSG00000130055
Entrez:   54857
UniGene:   Hs.438712
Chromosome Mapping:   Xq13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.940000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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