PrimePCR™ Probe Assay: MPDU1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051521
Assay Design:   exonic
Chromosome Location:   17:7489289-7490028question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000250124 ENST00000396501 ENST00000584378 ENST00000423172 ENST00000579445 ENST00000585217 ENST00000581380 ENST00000396573 ENST00000562109 ENST00000330684 ENST00000396575 ENST00000404927 ENST00000535259

Gene Information

This gene encodes an endoplasmic reticulum membrane protein that is required for utilization of the mannose donor mannose-P-dolichol in the synthesis of lipid-linked oligosaccharides and glycosylphosphatidylinositols. Mutations in this gene result in congenital disorder of glycosylation type If. Alternative splicing results in multiple transcript variants. [provided by RefSeq Dec 2008]

Gene Symbol:   MPDU1
Gene Name:   mannose-P-dolichol utilization defect 1
Aliases:   CDGIF, FLJ14836, HBEBP2BPA, Lec35, My008, PP3958, PQLC5, SL15
RefSeq:   NC_000017.10 NG_009204.1 NT_010718.16
Ensembl:   ENSG00000129255
Entrez:   9526
UniGene:   Hs.246381
Chromosome Mapping:   17p13.1-p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.880000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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