PrimePCR™ Probe Assay: INO80, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051498
Assay Design:   exonic
Chromosome Location:   15:41272235-41272352question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000361937 ENST00000401393

Gene Information

The protein encoded by this gene represents the catalytic ATPase subunit of the INO80 chromatin remodeling complex. This complex binds DNA and is recruited by YY1 to activate certain genes.[provided by RefSeq Apr 2010]

Gene Symbol:   INO80
Gene Name:   INO80 homolog (S. cerevisiae)
Aliases:   INO80A, INOC1, hINO80
RefSeq:   NC_000015.9 NG_017163.1 NT_010194.17
Ensembl:   ENSG00000128908
Entrez:   54617
UniGene:   Hs.292949
Chromosome Mapping:   15q15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 36.500000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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