This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: HAT1, Human
PrimePCR™ Template for Probe Assay: HAT1, Human
The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation particularly of histone H4 plays an important role in replication-dependent chromatin assembly. Specifically this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12 and to a lesser degree histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq Jun 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.