PrimePCR™ Probe Assay: FEZF1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051487
Assay Design:   exonic
Chromosome Location:   7:121943264-121943697question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000442488 ENST00000427185 ENST00000331178

Gene Information

Description Not Available

Gene Symbol:   FEZF1
Gene Name:   FEZ family zinc finger 1
Aliases:   FEZ, ZNF312B
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000128610
Entrez:   389549
UniGene:   Hs.553970
Chromosome Mapping:   7q31.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.340000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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