PrimePCR™ Probe Assay: SMO, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051485
Assay Design:   exonic
Chromosome Location:   7:128852969-128853111question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000249373

Gene Information

The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex. [provided by RefSeq Jul 2010]

Gene Symbol:   SMO
Gene Name:   smoothened homolog (Drosophila)
Aliases:   Gx, SMOH
RefSeq:   NC_000007.13 NG_023340.1 NT_007933.15
Ensembl:   ENSG00000128602
Entrez:   6608
UniGene:   Hs.437846
Chromosome Mapping:   7q32.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.370000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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