PrimePCR™ Probe Assay: GAL3ST1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GAL3ST1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GAL3ST1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051474
Assay Design:   exonic
Chromosome Location:   22:30951779-30951884question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000406955 ENST00000402321 ENST00000402369 ENST00000401975 ENST00000406361 ENST00000441967 ENST00000431313 ENST00000452827 ENST00000437282 ENST00000416358 ENST00000427899 ENST00000423299 ENST00000443136 ENST00000338911 ENST00000443111

Gene Information

Sulfonation an important step in the metabolism of many drugs xenobiotics hormones and neurotransmitters is catalyzed by sulfotransferases. The product of this gene is galactosylceramide sulfotransferase which catalyzes the conversion between 3'-phosphoadenylylsulfate + a galactosylceramide to adenosine 3'5'-bisphosphate + galactosylceramide sulfate. Activity of this sulfotransferase is enhanced in renal cell carcinoma. [provided by RefSeq Jul 2008]

Gene Symbol:   GAL3ST1
Gene Name:   galactose-3-O-sulfotransferase 1
Aliases:   CST
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000128242
Entrez:   9514
Chromosome Mapping:   22q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.950000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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