PrimePCR™ Probe Assay: AKAP9, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AKAP9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AKAP9, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051457
Assay Design:   exonic
Chromosome Location:   7:91714247-91714915question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000358100 ENST00000359028 ENST00000435423 ENST00000394534 ENST00000356239

Gene Information

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternate splicing of this gene results in at least two isoforms that localize to the centrosome and the Golgi apparatus and interact with numerous signaling proteins from multiple signal transduction pathways. These signaling proteins include type II protein kinase A serine/threonine kinase protein kinase N protein phosphatase 1 protein phosphatase 2a protein kinase C-epsilon and phosphodiesterase 4D3. [provided by RefSeq Aug 2008]

Gene Symbol:   AKAP9
Gene Name:   A kinase (PRKA) anchor protein (yotiao) 9
Aliases:   AKAP-9, AKAP350, AKAP450, CG-NAP, HYPERION, KIAA0803, MU-RMS-40.16A, PRKA9, YOTIAO
RefSeq:   NG_007968.1 NC_000007.13 NG_011623.1 NT_007933.15
Ensembl:   ENSG00000127914
Entrez:   10142
Chromosome Mapping:   7q21-q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.810000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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