PrimePCR™ Probe Assay: AIF1L, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051424
Assay Design:   exonic
Chromosome Location:   9:133995738-133995832question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000322644 ENST00000247291 ENST00000372302 ENST00000372300 ENST00000372301 ENST00000372309 ENST00000372312 ENST00000311875 ENST00000512012 ENST00000438774

Gene Information

Description Not Available

Gene Symbol:   AIF1L
Gene Name:   allograft inflammatory factor 1-like
Aliases:   C9orf58, FLJ12783, IBA2, MGC29466
RefSeq:   NC_000009.11 NG_023371.1 NT_035014.4
Ensembl:   ENSG00000126878
Entrez:   83543
UniGene:   Hs.4944
Chromosome Mapping:   9q34.13-q34.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.830000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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