PrimePCR™ Probe Assay: DEFB129, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051384
Assay Design:   exonic
Chromosome Location:   20:210267-210416question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000246105

Gene Information

Defensins are cysteine-rich cationic polypeptides that are important in the immunologic response to invading microorganisms. The protein encoded by this gene is secreted and is a member of the beta defensin protein family. Beta defensin genes are found in several clusters throughout the genome with this gene mapping to a cluster at 20p13. [provided by RefSeq Jul 2008]

Gene Symbol:   DEFB129
Gene Name:   defensin, beta 129
Aliases:   C20orf87, DEFB-29, DEFB29, bA530N10.3, hBD-29
RefSeq:   NC_000020.10 NT_011387.8
Ensembl:   ENSG00000125903
Entrez:   140881
UniGene:   Hs.112087
Chromosome Mapping:   20p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.600000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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