PrimePCR™ Probe Assay: DTD1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051368
Assay Design:   exonic
Chromosome Location:   20:18744330-18744469question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000377452

Gene Information

The protein encoded by this gene is similar in sequence to histidyl-tRNA synthetase which hydrolyzes D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr). The encoded protein is found in the cytoplasm. [provided by RefSeq Jul 2008]

Gene Symbol:   DTD1
Gene Name:   D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae)
Aliases:   C20orf88, DUEB, HARS2, MGC119131, MGC41905, bA379J5.3, bA555E18.1, pqn-68
RefSeq:   NC_000020.10 NT_011387.8
Ensembl:   ENSG00000125821
Entrez:   92675
UniGene:   Hs.659442
Chromosome Mapping:   20p11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.290000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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