PrimePCR™ Probe Assay: POLR1B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: POLR1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: POLR1B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051353
Assay Design:   exonic
Chromosome Location:   2:113300093-113300229question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000263331 ENST00000438748 ENST00000430769 ENST00000409894 ENST00000417433 ENST00000541869 ENST00000537335

Gene Information

Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt 1996 [PubMed 8921381]).[supplied by OMIM Mar 2008]

Gene Symbol:   POLR1B
Gene Name:   polymerase (RNA) I polypeptide B, 128kDa
Aliases:   FLJ10816, FLJ21921, MGC131780, RPA135, RPA2, Rpo1-2
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000125630
Entrez:   84172
Chromosome Mapping:   2q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.530000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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