PrimePCR™ Probe Assay: CNOT1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051336
Assay Design:   exonic
Chromosome Location:   16:58633214-58633340question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000317147 ENST00000569240 ENST00000441024 ENST00000564557 ENST00000565697 ENST00000569020

Gene Information

Description Not Available

Gene Symbol:   CNOT1
Gene Name:   CCR4-NOT transcription complex, subunit 1
Aliases:   CDC39, DKFZp686E0722, DKFZp686O168, FLJ36492, FLJ90644, KIAA1007, NOT1, NOT1H
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000125107
Entrez:   23019
UniGene:   Hs.460923
Chromosome Mapping:   16q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.590000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download