PrimePCR™ Probe Assay: ZNF45, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051304
Assay Design:   exonic
Chromosome Location:   19:44419133-44419281question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000269973 ENST00000589703 ENST00000588140

Gene Information

Description Not Available

Gene Symbol:   ZNF45
Gene Name:   zinc finger protein 45
Aliases:   KOX5, ZNF13
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000124459
Entrez:   7596
UniGene:   Hs.381285
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 38.440000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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