PrimePCR™ Probe Assay: SARDH, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051251
Assay Design:   exonic
Chromosome Location:   9:136596505-136596598question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000371872 ENST00000427237 ENST00000371867 ENST00000439388 ENST00000422262 ENST00000298628

Gene Information

This gene encodes an enzyme localized to the mitochondrial matrix which catalyzes the oxidative demethylation of sarcosine. This enzyme is distinct from another mitochondrial matrix enzyme dimethylglycine dehydrogenase which catalyzes a reaction resulting in the formation of sarcosine. Mutations in this gene are associated with sarcosinemia. Alternatively spliced transcript variants have been described. [provided by RefSeq Oct 2008]

Gene Symbol:   SARDH
Gene Name:   sarcosine dehydrogenase
Aliases:   DMGDHL1, FLJ36475, SAR, SARD, SDH
RefSeq:   NC_000009.11 NT_035014.4 NG_008987.1
Ensembl:   ENSG00000123453
Entrez:   1757
UniGene:   Hs.198003
Chromosome Mapping:   9q33-q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.440000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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