This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: ATP7B, Human
PrimePCR™ Template for Probe Assay: ATP7B, Human
This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains an ATPase consensus sequence a hinge domain a phosphorylation site and at least 2 putative copper-binding sites. This protein functions as a monomer exporting copper out of the cells such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants encoding different isoforms with distinct cellular localizations have been characterized. Mutations in this gene have been associated with Wilson disease (WD). [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.