PrimePCR™ Probe Assay: POLM, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051219
Assay Design:   exonic
Chromosome Location:   7:44120335-44121869question
Amplicon Length:   171
Splice Variants Targeted:   ENST00000335195 ENST00000242248 ENST00000395831 ENST00000414235 ENST00000452049

Gene Information

Description Not Available

Gene Symbol:   POLM
Gene Name:   polymerase (DNA directed), mu
Aliases:   FLJ35482, Pol Mu, Tdt-N
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000122678
Entrez:   27434
UniGene:   Hs.596982
Chromosome Mapping:   7p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.030000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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