PrimePCR™ Probe Assay: OCM, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051209
Assay Design:   exonic
Chromosome Location:   7:5920429-5920543question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000216064 ENST00000405018 ENST00000405510 ENST00000332303 ENST00000257627 ENST00000360639 ENST00000394987 ENST00000567617 ENST00000302177 ENST00000361722 ENST00000242104 ENST00000307271 ENST00000377996 ENST00000439587 ENST00000377991 ENST00000377988

Gene Information

Oncomodulin is a high-affinity calcium ion-binding protein. It belongs to the superfamily of calmodulin proteins also known as the EF-hand proteins. Oncomodulin is an oncodevelopmental protein found in early embryonic cells in the placenta and also in tumors. [provided by RefSeq Jul 2008]

Gene Symbol:   OCM
Gene Name:   oncomodulin
Aliases:   MGC142264, MGC142266, MGC97453, MGC97465, MGC97477, OCM1, OM, ONCM
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000122543
Entrez:   654231
UniGene:   Hs.571315
Chromosome Mapping:   7p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.000000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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