PrimePCR™ Probe Assay: POLK, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051188
Assay Design:   exonic
Chromosome Location:   5:74865181-74865301question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000241436 ENST00000514296 ENST00000515295 ENST00000504026 ENST00000508526 ENST00000352007 ENST00000380481

Gene Information

External and internal DNA-damaging agents continually threaten the integrity of genetic material in cells. Although a variety of repair mechanisms exist to remove the resulting lesions some lesions escape repair and block the replication machinery. Members of the Y family of DNA polymerases such as POLK permit the continuity of the replication fork by allowing replication through such DNA lesions. Each Y family polymerase has a unique DNA-damage bypass and fidelity profile. POLK is specialized for the extension step of lesion bypass (summary by Lone et al. 2007 [PubMed 17317631]).[supplied by OMIM Jan 2010]

Gene Symbol:   POLK
Gene Name:   polymerase (DNA directed) kappa
Aliases:   DINB1, DINP, POLQ
RefSeq:   NC_000005.9 NT_006713.15
Ensembl:   ENSG00000122008
Entrez:   51426
UniGene:   Hs.732105
Chromosome Mapping:   5q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.850000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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